γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation
نویسنده
چکیده
γδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2– subsets of γδ-TCR lymphocytes were analyzed. CD2+ γδ-TCR lymphocytes markedly prevailed over CD2– cells in intact mammary gland: 88.9 ± 4.9% of γδ-TCR lymphocytes were CD2+. After stimulation, neutrophils and γδ-TCR lymphocytes were recruited into the mammary gland. Among γδ-TCR lymphocytes, CD2– cells were mainly responsible for their expansion. After stimulation, 60.8 ± 13.4% of γδ-TCR lymphocytes were CD2+ (P < 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland.
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